Epithalon 50mg Reconstitution and Research Protocol

Epithalon Research Reconstitution Protocol

Epithalon (Ala-Glu-Asp-Gly, MW ~432.4 Da) is supplied as a white lyophilized powder. Its small tetrapeptide structure makes it highly stable and relatively easy to reconstitute compared to larger peptides.

Materials

• Epithalon 50mg lyophilized vial
• Bacteriostatic water (0.9% benzyl alcohol)
• Sterile insulin syringe (28–31 gauge)
• Alcohol swabs
• Amber vial or light-protective storage container

Concentration Options for 50mg Vial

BAC Water Added

Concentration

Per 0.1mL

5 mL	10 mg/mL	1.0 mg
10 mL	5 mg/mL	0.5 mg
25 mL	2 mg/mL	0.2 mg
50 mL	1 mg/mL	0.1 mg

The 5mg/mL concentration (10mL BAC water) is commonly used in research settings for convenient dosing calculations.

Reconstitution Steps

Step 1: Prepare Workspace

Wipe all surfaces. Use gloves. Allow vial to equilibrate to room temperature (15–20 min from refrigerator).

Step 2: Inspect Lyophilized Peptide

Epithalon lyophilized cake should be white, uniform, and fluffy. Any yellow discoloration or condensation inside the sealed vial suggests improper storage.

Step 3: Add Bacteriostatic Water

• Wipe vial stopper with 70% alcohol swab
• Draw calculated BAC water volume into syringe
• Insert needle bevel-up at 45°
• Slowly inject down glass wall — do not jet directly onto peptide cake
• Remove syringe

Step 4: Dissolve

Epithalon dissolves quickly (30–60 seconds) due to its small MW. Gently swirl. Do not vortex. Solution should be clear and colorless.

Storage After Reconstitution

• Refrigerator (2–8°C): Stable approximately 6–8 weeks in BAC water
• Freezer (−20°C): Suitable for lyophilized form only; avoid freeze-thaw cycles with reconstituted peptide
• Protect from UV light; use amber vials or foil wrapping

Stability Notes

Epithalon's tetrapeptide structure is notably stable:

• pH stability: 4–8 range
• Thermal stability: Lyophilized form stable at room temperature short-term
• Reconstituted: More sensitive — maintain cold chain

For laboratory research use only. Not for human administration.